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Quick protein binding analysis by label-free thermal shift analysis on the Tycho NT.6

The lock-and-key model first postulated by the famous chemist Emil Fischer proposes that enzymes and their substrates must fit together like locks and keys in order to fulfill their physiological functions. Even though it has long become clear that biological systems are nowhere near as rigid as their metallic namesakes, the principle of molecules interacting together three-dimensionally is the basis for almost all biological processes. Of course this is not only true for enzymes, but for all types of protein interactions. Only a protein that is correctly folded and structurally intact can do its job properly. For researchers working with proteins, it is therefore fundamentally important to be able to analyze both protein structure and function.

To determine whether a protein is functional and able to bind its interaction partner or ligand, Tycho NT.6 can examine protein-ligand interactions by performing a label-free thermal shift analysis. Suitable ligands can be varied and diverse, ranging from ions, sugars and small molecules to nucleotides and lipids. By recording and comparing highly precise unfolding profiles, ligand-induced changes in the protein’s stability as monitored by fluorescence properties can be derived to conclude binding. Thus, the Tycho NT.6 system allows for evaluating protein functionality within minutes, and can be integrated in purification workflows or assay development routines with ease.

Validation of protein complex functionality with Tycho NT.6
Validation of protein complex functionality with Tycho NT.6

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Better optimization of biosensor assay development with Tycho NT.6
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