Monolith Publications

  • MST and TRIC technology to reliably study PROTAC binary and ternary binding in drug development

    MST and TRIC technology to reliably study PROTAC binary and ternary binding in drug development

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  • Ddi1 is a ubiquitin-dependent protease

    Ddi1 is a ubiquitin-dependent protease

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  • Quantifying the interaction of phosphite with ABC transporters: MicroScale Thermophoresis and a novel his-tag labeling approach

    Quantifying the interaction of phosphite with ABC transporters: MicroScale Thermophoresis and a novel his-tag labeling approach

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  • Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity nanobody

    Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity nanobody

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  • Sweet and blind spots in E3 ligase ligand space revealed by a thermophoresis-based assay

    Sweet and blind spots in E3 ligase ligand space revealed by a thermophoresis-based assay

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  • Lipids activate SecA for high affinity binding to the SecYEG complex

    Lipids activate SecA for high affinity binding to the SecYEG complex

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  • Ready to characterize your most challenging interactions?

    Discover tools to measure binding affinity

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  • Thermophoretic analysis of ligand-specific conformational states of the inhibitory glycine receptor embedded in copolymer nanodiscs

    Thermophoretic analysis of ligand-specific conformational states of the inhibitory glycine receptor embedded in copolymer nanodiscs

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  • Detergent-free extraction of a functional low-expressing GPCR from a human cell line

    Detergent-free extraction of a functional low-expressing GPCR from a human cell line

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  • Phospholipid membrane fluidity alters ligand binding activity of a G protein-coupled receptor by shifting the conformational equilibrium

    Phospholipid membrane fluidity alters ligand binding activity of a G protein-coupled receptor by shifting the conformational equilibrium

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  • Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5

    Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5

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  • Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions

    Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions

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  • Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1

    Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1

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  • Targeting the SARS-CoV‑2 RNA genome with small molecule binders and ribonuclease targeting chimera (RIBOTAC) degraders

    Targeting the SARS-CoV‑2 RNA genome with small molecule binders and ribonuclease targeting chimera (RIBOTAC) degraders

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  • Targeted degradation of a hypoxia-associated non-coding RNA enhances the selectivity of a small molecule interacting with RNA

    Targeted degradation of a hypoxia-associated non-coding RNA enhances the selectivity of a small molecule interacting with RNA

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  • The peptide PROTAC modality: a novel strategy for targeted protein ubiquitination

    The peptide PROTAC modality: a novel strategy for targeted protein ubiquitination

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  • A cell-permeable peptide-based PROTAC against the oncoprotein CREPT proficiently inhibits pancreatic cancer

    A cell-permeable peptide-based PROTAC against the oncoprotein CREPT proficiently inhibits pancreatic cancer

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  • High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from Coronavirus 2

    High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from Coronavirus 2

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  • Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing

    Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing

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  • Antibody-targeted chromatin enables effective intracellular delivery and functionality of CRISPR/Cas9 expression plasmids

    Antibody-targeted chromatin enables effective intracellular delivery and functionality of CRISPR/Cas9 expression plasmids

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  • Spatial screening of hemagglutinin on Influenza A Virus particles: Sialyl-LacNAc displays on DNA and PEG scaffolds reveal requirements

    Spatial screening of hemagglutinin on Influenza A Virus particles: Sialyl-LacNAc displays on DNA and PEG scaffolds reveal requirements

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