Membrane Proteins
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ReadOptimization of membrane proteins for cryo-EM: Screening of copolymer nanodiscs with Prometheus Panta
Membrane proteins present challenges for structural analysis by cryo-EM; Screening of synthetic copolymers with nanoDSF and DLS on Prometheus Panta help select the best solubilization conditions.
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20:31WatchThe power of Monolith X for Characterizing GPCRs: Accelerating Binding Affinity Measurements
To understand signaling pathways, Spectral Shift technology allows for accurate bindings affinity measurements of membrane proteins like GPCRs in close to native conditions.
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14:01WatchHow to study molecular interactions of membrane proteins without compromising their native structure
The desire to use technology that allows you to study membrane proteins in close-to-native conditions is specifically important when it comes to interaction measurements. It’s always a question how mu
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Working with tricky membrane proteins?
Find out how
NanoTemper tools can help -
12:06WatchHow to find expression and purification conditions that result in high levels of thermally stable membrane proteins
What you’ll learn It’s very common to experience low expression levels when producing membrane proteins — plus they’re finicky and unstable when they're outside of their native environment. Just know
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4:09WatchDid you say, “membrane proteins”? Challenge accepted!
Working with difficult targets like GPCRs and other membrane proteins? Because they live in the cell membrane, are hydrophobic and insoluble, have low expression levels, and require particular deter
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25:40WatchMischievous membrane proteins (and how to tame them)
Are you struggling to tame your mischievous membrane proteins? Find out how NanoTemper can help you to characterize even the most challenging membrane protein samples. You will learn how Prometheus ca
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ReadQuantifying the interaction of phosphite with ABC transporters: MicroScale Thermophoresis and a novel his-tag labeling approach
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nanoDSF: Label-free thermal unfolding assay of G Protein-coupled receptors for compound screening and buffer composition optimization
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ReadLipids activate SecA for high affinity binding to the SecYEG complex
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ReadThermophoretic analysis of ligand-specific conformational states of the inhibitory glycine receptor embedded in copolymer nanodiscs
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ReadA protein superfamily meets its match in nanoDSF
G protein-coupled receptors (GPCRs) are the largest class of targets for drug discovery, and the fourth largest superfamily in the human genome. They have been linked to the regulation of many...
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ReadDetergent-free extraction of a functional low-expressing GPCR from a human cell line
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ReadSaposin lipid nanoparticles: A highly versatile and modular tool for membrane protein research
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ReadPhospholipid membrane fluidity alters ligand binding activity of a G protein-coupled receptor by shifting the conformational equilibrium
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ReadPhosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5
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Interactions of liposome embedded SNARE proteins
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ReadMicroscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
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ReadMolecular basis of nitrate uptake by the plant nitrate transporter NRT1.1
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ReadStructure of DtpA from E. coli in complex with Valganciclovir provides insights into drug binding of human PepT1
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ReadStructural basis of species-selective antagonist binding to the succinate receptor
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