Surface plasmon resonance (SPR) is an optical methodology used to detect and quantify molecular interactions. Although SPR is widely used and considered by many researchers to be the gold standard for quantification of protein interactions, assay optimization can be technically challenging, time-consuming and costly. The Tycho NT.6 system quickly analyzes different conditions typically tested when optimizing an SPR assay. It utilizes very small amounts of sample and provides dramatic time and cost savings compared to standard SPR assay development procedures. Tycho NT.6 is simple to use and enables researchers to make better educated decisions in developing and optimizing their binding interaction assays.
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Analyze self-interaction to predict viscosity and aggregation propensity at higher concentrations
Fast expression screening that reveals expression levels of properly folded recombinant proteins
Measure binding affinities for any type of interaction — examine a label-free or labeled target molecule at anytime
Accelerate your assay development using the enhanced Binding Check module in Monolith's MO.Control 2 software
Site-specific covalent labeling of SNAP-tagged proteins for the measurement of binding affinities
Protein labeling – improved quantitation of biomolecular interactions by MST using the His-Tag labeling kit RED-tris-NTA 2nd generation