How to improve the function of your PROTAC degrader by understanding ternary complex formation

In this video, Alessio Ciulli, Ph.D. shows how a PROTAC can “glue” the ligase and the target protein together by forming ternary complexes. He then demonstrates that cooperative, stable, and kinetically long-lived ternary complexes drive faster and more profound protein degradation.

Watch this video to learn the answers to these questions:

- How do PROTACs work?
- Why does the ternary complex matter?
- What defines cooperativity in complex formation?
- How does ternary complex formation inform rational structure-based drug design?
- How can ternary complex optimization help overcome challenges with delivering the PROTAC to the cell?

NanoTemper tools are helping researchers characterize interactions involving PROTACs, molecular glues and other TACs.

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Sweet and blind spots in E3 ligase ligand space revealed by a thermophoresis-based assay
Sweet and blind spots in E3 ligase ligand space revealed by a thermophoresis-based assay

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