p38α is considered as the key isoform involvedin modulating inflammatory response inrheumatoid arthritis and inflammatory painand is therefore a key target for compoundscreening in pharmaceutical industry. In thisstudy we demonstrate the use of MicroscaleThermophoresis to perform an essentiallylabel-free measurements of small moleculebinding to protein targets by using acompetitive approach. This approach thereforeallows screening projects aiming to identifyand characterize site-specific binders orbinders allosterically influencing a specificbinding site out of a compound library. Furthermore this approach also can be used toscreen for compounds that interrupt proteinprotein,protein-peptide or protein-nucleic acidinteractions.
Home
»
Application Notes
»
Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38
×
Share
Have a question about Monolith?
Contact SpecialistMost recent content
How Merck KGaA uses light scattering measurements for better developability assessment of biologic therapeutics
Biologics developability: learn how Merck uses Prometheus Panta to create developability profiles for their mAbs
Quantifying oligonucleotides binding to human serum albumin with MST: A faster and precise approach for drug candidates ADME profiling
Fast molecular interaction screening of epigenetic gene regulator G9a with fragments from a large chemical space
Rapid Quantification of Unfolded Proteins for Quality Control and Optimization of Storage Conditions
Protein labeling – improved quantitation of biomolecular interactions by MST using the RED-NHS 2nd generation labeling kit
One-step, purification-free and site-specific labeling of polyhistidine-tagged proteins for MST
Anaerobic MicroScale Thermophoresis reveals the redox dependency of ferredoxin in mitochondrial Fe/S biogenesis
The decondensation factor 31 specifically interacts with histones H3 and H4 but not H2A and H2B