Membrane proteins are critical in drug discovery due to their central role in cellular communication and signaling, making them key therapeutic targets for next generation medicines. However, their unique properties pose significant challenges for biophysical characterization, particularly in maintaining stability and native conformation outside of biological membranes. The stringent buffer conditions required to maintain the integrity of membrane proteins when investigating their structure and function are limiting, highlighting the need for innovative technologies that don’t rely on traditional detergent-based approaches to facilitate novel drug discovery and drug interaction analysis with membrane proteins.
This application note underscores the combined strength of using the latest technologies from Cube Biotech and NanoTemper Technologies to supersede these challenges. NativeMP™ Technology, developed by Cube Biotech, utilizes detergent-free synthetic copolymers to stabilize membrane proteins in their native environment, while the Monolith X instrument from NanoTemper Technologies employs Spectral Shift and TRIC technologies to directly measure biomolecular interactions in solution; coupling these technologies empowers researchers to obtain highly sensitive and precise binding affinities while preserving the protein’s native conformation during measurements.
Learn more about Monolith X in this short demo video.