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Interactions of Liposome embedded SNARE Proteins

SNARE protein complexes play a key role in membrane fusion processes, e.g. the fusion of synaptic vesicles with the pre-synaptic plasma membrane in response to elevated Ca2+ levels. This study aimed to demonstrate the use of Microscale Thermophoresis for the detection of protein-protein interaction in more complex assay systems like in this case the interaction of membrane proteins embedded into liposomes.

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Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38
Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38

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Binding of the geldanamycin derivative 17 DMAG to Hsp90 measured with fluorescence label and label free
Binding of the geldanamycin derivative 17 DMAG to Hsp90 measured with fluorescence label and label free

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