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Binding of calcium ions to synaptotagmin measured with fluorescence label and label-free

The synaptic vesicle protein synaptotagmin 1 is the main calcium sensor of neuronal exocytosis. Calcium binds to its cytosolic portion that consists of tandem C2-type domains. In this work we show that Microscale Thermophoresis is a valuable tool to measure binding of ions to proteins, with and without the use of a fluorescent label.

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Binding of histone peptides to chromatin assembly factor I (CAF-I) p48 subunit
Binding of histone peptides to chromatin assembly factor I (CAF-I) p48 subunit

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Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38
Competitive Assay Approach: Binding of Small Molecules to the Active Form of p38

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