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Binding of histone peptides to chromatin assembly factor I (CAF-I) p48 subunit

p48, the small subunit of chromatin assemblyfactor 1 (CAF-1), is a member of a highly conserved subfamily of WD-repeat proteins. There are at least two members of this subfamilyin human (p46 and p48). p48 copurifies with a chromatin assembly complex (CAC), which contains the three subunits of CAF-1 (p150, p60,p48) and the Histones H3 and H4, and promotesDNA replication-dependent chromatin assembly. In this study we analyze the binding of H3 and H4 peptides to p48 using MicroscaleThermophoresis (MST). The study also highlights the high content information of the MST measurements as one important benefit of Microscale Thermophoresis.

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Using MST to analyse the binding of nanobodies and nanobody-Fc fusion proteins to human CD38
Using MST to analyse the binding of nanobodies and nanobody-Fc fusion proteins to human CD38

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