SNARE protein complexes play a key role in membrane fusion processes, e.g. the fusion of synaptic vesicles with the pre-synaptic plasma membrane in response to elevated Ca2+ levels. This study aimed to demonstrate the use of Microscale Thermophoresis for the detection of protein-protein interaction in more complex assay systems like in this case the interaction of membrane proteins embedded into liposomes.
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14:01The desire to use technology that allows you to study membrane proteins in close-to-native conditions is specifically important when it comes to interaction measurements. It’s always a question how mu
12:06What you’ll learn It’s very common to experience low expression levels when producing membrane proteins — plus they’re finicky and unstable when they're outside of their native environment. Just know
4:09Working with difficult targets like GPCRs and other membrane proteins? Because they live in the cell membrane, are hydrophobic and insoluble, have low expression levels, and require particular deter
25:40Are you struggling to tame your mischievous membrane proteins? Find out how NanoTemper can help you to characterize even the most challenging membrane protein samples. You will learn how Prometheus ca
Quantifying the interaction of phosphite with ABC transporters: MicroScale Thermophoresis and a novel his-tag labeling approach
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A protein superfamily meets its match in nanoDSF
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Phospholipid membrane fluidity alters ligand binding activity of a G protein-coupled receptor by shifting the conformational equilibrium
Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5
Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
Structure of DtpA from E. coli in complex with Valganciclovir provides insights into drug binding of human PepT1
Towards high throughput GPCR crystallography: In Meso soaking of Adenosine A2A Receptor crystals