SNARE protein complexes play a key role in membrane fusion processes, e.g. the fusion of synaptic vesicles with the pre-synaptic plasma membrane in response to elevated Ca2+ levels. This study aimed to demonstrate the use of Microscale Thermophoresis for the detection of protein-protein interaction in more complex assay systems like in this case the interaction of membrane proteins embedded into liposomes.
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Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5
Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
Structure of DtpA from E. coli in complex with Valganciclovir provides insights into drug binding of human PepT1
Towards high throughput GPCR crystallography: In Meso soaking of Adenosine A2A Receptor crystals
Lipid-like peptides can stabilize integral membrane proteins for biophysical and structural studies
Structural basis for substrate specificity and regulation of nucleotide sugar transporters in the lipid bilayer
Membrane (and soluble) protein stability and binding measurements in the lipid cubic phase using label-free DSF
