Selecting the optimum method for binding measurements is crucial for obtaining reliable results and these are essential for advancing your projects in drug discovery and cellular pathway studies. Therefore, careful consideration is required when planning experiments. Biophysical techniques for affinity determination fall into three categories: label-free, immobilization-based, and fluorescence-based methods, each offering distinct advantages in terms of throughput and target/ligand modifications. For free in-solution measurements, preserving the native state of molecules, and achieving high sensitivity, fluorescence-based approaches are ideal. Regardless of the specific fluorescence technology (e.g., Spectral Shift, TRIC, or FRET), optimizing your labeling strategy is essential to minimize impact on molecular functionality and ensure high-quality, reproducible data for accurate Kd characterization.
Join this webinar to learn:
- How fluorescence-based technologies like Spectral Shift and its fluorescent labels are designed to measure affinities directly in solution
- Best practices for selecting labeling strategies
- Why in-solution, fluorescence-based measurements offer greater flexibility for assays
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