Protein purification-free binding analysis by MicroScale Thermophoresis

Microscale thermophoresis (MST) can be widely used for determination of binding affinity without purification of the target protein from cell lysates. The protocol involves overexpression of the GFP-fused protein, cell lysis in non-denaturing conditions, and detection of MST signal in the presence of varying concentrations of the ligand.

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Monolith NT.Automated: discover the fully automated MST performance
Monolith NT.Automated: discover the fully automated MST performance

MicroScale Thermophoresis (MST) is an easy, fast and precise way to quantify biomolecular interactions. Wit...

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Microscale Thermophoresis - a technology by NanoTemper
Microscale Thermophoresis - a technology by NanoTemper

Microscale Thermophoresis Technology (MST) is based on a physical principle used for the first time in biom...