Here we report a thermodynamic analysis of biomolecular interactions using MicroScale Thermophoresis (MST). Using DNA hybridization with fluorescently labeled oligonucleotides as a well-characterized model system, we monitor the shift in the dissociation (Kd) constant of the interaction over a range of temperatures and calculate the free enthalpy (ΔH) and entropy (ΔS) for the hybridization reaction. The obtained values were in excellent agreement with theoretically calculated values, demonstrating that MST is feasible to perform thermodynamic analysis of biomolecules.
Have a question about Monolith?
Contact SpecialistSee more related content
15:19Virtual Monolith X Demo: Free-in-solution affinity measurements with minimal sample consumption
The tool you choose to measure affinities should tackle a diverse range of targets and ligands that come your way — such as GPCRs, IDPs, or cancer-related proteins. And, you should get data that help
20:31The power of Monolith X for Characterizing GPCRs: Accelerating Binding Affinity Measurements
To understand signaling pathways, Spectral Shift technology allows for accurate bindings affinity measurements of membrane proteins like GPCRs in close to native conditions.
Three things we took away from attending Drug Discovery Chemistry in 2024
Drug Discovery Chemistry is an annual event that focuses solely on early drug discovery, where scientists from around the world gather to showcase their research and participate in thoughtful...
NanoTemper Technologies introduced Monolith X, adding breakthrough Spectral Shift technology to their top-selling Monolith product line.
2:20Introducing Monolith X - the only way to get binding affinities in solution using very minimal sample
NanoTemper’s Monolith wins top global design award: Red Dot Award
NanoTemper’s newest Monolith instrument has been awarded the Red Dot Award, an internationally recognized seal of excellence in design. Both bold aesthetics and user-friendly design have always...
10 terms to help you more easily understand targeted protein degradation
If you’re new to the world of TPD or you’re trying to keep up with all developments in this fast-moving field, here are some terms and definitions that will help you.
14:01The desire to use technology that allows you to study membrane proteins in close-to-native conditions is specifically important when it comes to interaction measurements. It’s always a question how mu
35:47Targeted protein degradation using molecular glues or proteolysis-targeting chimeras (PROTACs) is an increasingly important therapeutic modality, especially for undruggable targets. Even with candidat
Why so many companies are betting on PROTACs as a superior therapeutic modality
One advantage of PROTACs is their unique mode of action — the induced proximity of an E3 ligase and the protein of interest (POI) triggers the ubiquitination and then degradation of the POI by the...
35:24Effect of different SARS-CoV-2 variants on COVID 19 assays and vaccines
SARS-CoV-2, the causative agent of COVID-19, has an ssRNA genome of almost 30kb that encodes for a total of 26 proteins. Among the four structural viral protein, in particular the S protein stands out
MST and TRIC technology to reliably study PROTAC binary and ternary binding in drug development
27:20Explore the complexities and drivers of molecular interactions with Dr. Tom Laue from the University of New Hampshire. He presented as a keynote speaker in the Protein Stability & Function: Can't have
33:11Dr. Leah Pandiscia and Dr. Daniel Turman review how to integrate the Prometheus Panta and Monolith instruments in a characterization workflow demonstration, showing how to maximize stability and funct
NanoTemper’s Monolith wins top global design award: iF DESIGN AWARD 2021
NanoTemper’s newest Monolith instrument has been awarded the globally renowned iF DESIGN AWARD 2021. User-friendly design has always been a core focus in NanoTemper’s product development....