Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry

November 30, 2020

Popp, C., Schramm, P., Wagner, R., et al.

bioRxiv 2020, doi: 10.1101/2020.11.30.403873

Abstract

Vaccines are on the front line-of-defense against infectious diseases, ranging from threats we are familiar with, including polio, tuberculosis, or HIV, to novel and emerging threats such as SARS-CoV-2. Successful development of new protein-based vaccines requires sophisticated and efficient development of storage and formulation conditions. Here we demonstrate the combined power of 2bind’s sophisticated buffer matrix FORMOscreen® and NanoTemper Technologies’ novel Prometheus Panta high-throughput Dynamic Light Scattering/Nano Differential Scanning Fluorimetry instrument. We show that this combination can comprehensively improve critical biophysical parameters for the HIV-1 vaccine BG505-SOSIP and find the optimal formulation condition with unmatched efficiency.

View Publication

Topics: Prometheus, nanoDSF, DLS, Biologics, Publications

Previous Article
Exploring chemical space for new substances to stabilize a therapeutic monoclonal antibody
Exploring chemical space for new substances to stabilize a therapeutic monoclonal antibody

Up next
Predicting antibody developability profiles through early stage discovery screening
Predicting antibody developability profiles through early stage discovery screening

Looking for the optimal formulation for your biologics candidates?

NanoTemper tools can help

Find out how