Join Dr. Thomas Schubert, CEO of 2bind, as he walks through information from the Prometheus Panta that helped them select the optimal buffer for a vaccine antigen.
You’ll learn about:
- Why 2bind required both thermal and colloidal stability information to identify the optimal buffer for antigen stability out of a 96 condition screen
- Which stress conditions they assessed to probe the limits of antigen stability in the selected buffer, and how DLS helped them do it
- How they further used DLS to validate epitope integrity and antibody binding to the antigen
- How the group used a single instrument to conduct all their experiments for buffer optimization
Why it’s important for vaccine development:
Stable target antigens are a prerequisite for studying viruses and developing effective vaccines, but protein-based antigens don’t always remain well-folded and functional long-term.
Buffer optimization is a great approach for improving antigen stability. However, with hundreds to thousands of conditions to screen it’s critical to maximize the data you gather from any single stability experiment to facilitate your decision-making process.
Use a single instrument to get information on the thermal and colloidal stability of your antigen in different buffers to help you select one that is ideal for experimental and storage conditions.
Want to know about high precision tools for the combined characterization of thermal and colloidal stability of biologics? Learn more about Prometheus Panta