Chemical and thermal stability screening of an IgG1-antibody

November 10, 2018

The main purpose in formulation development of biologicals, such as monoclonal antibodies, is to establish optimal conditions for long-term stability of the protein. 

Here we used the Prometheus NT.48 instrument by NanoTemper Technologies to perform a buffer screening for an IgG1-Antibody. This instrument uses nanoDSF technology to measure thermal and chemical stability as indicators to predict the best buffer conditions for the protein. 

The results show that thermal and chemical unfolding, together with short-term stability studies, provides a complementary screening tool for finding optimal buffer conditions.

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Formulation and stability screening of biotherapeutics
Formulation and stability screening of biotherapeutics

The stability of biopharmaceuticals is a complex mixture of parameters and plays a crucial role throughout ...

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nanoDSF thermal unfolding analysis of proteins without tryptophan residues
nanoDSF thermal unfolding analysis of proteins without tryptophan residues

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