The autocorrelation function: the key to understanding your biological molecules

Dynamic Light Scattering (DLS) is a technique that allows researchers to accurately size their biologic molecules and determine the distribution of particle sizes in a solution. In order to turn scattering information from a system into usable data for decision making, DLS software uses an autocorrelation function (ACF). 

The ACF can often seem like a black box for researchers, but understanding how it works and what a good fit looks like is crucial for data analysis. Both experts and novices can benefit from a review of how the ACF is generated and how to assess data quality. In this seminar, you will learn:

  • What is DLS (a brief overview)
  • What DLS information goes into an ACF
  • How an ACF is generated
  • What the different fit models mean for your samples
  • What impact contaminants and poor sample prep can have on your ACF data
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