Predicting antibody developability profiles through early stage discovery screening

Bailly, M., Mieczkowski, C., Juan, V., et al.

mAbs 2020, vol: 12(1) doi: 10.1080/19420862.2020.1743053

In this publication, the protein sciences group at Merck & Co. evaluates 152 human or humanized mAbs for their developability properties. They review the various developability properties they measure, as well as how they measure them. They then present two specific cases where early developability parameters for physicochemical properties correlate well with downstream success. 

4 key takeaways from this publication:

1. High-throughput methods for developability assessment combined with in silico predictive tools are required for the future of antibody development

2. Some of the most straightforward assays that gave the most developability information without using a lot of material included: size exclusion chromatrography (SEC), nano-Differential Scanning Fluorimetry (nanoDSF), and Affinity-capture self-interaction nanoparticle spectroscopy (AC-SINS)

3. Humanizing one of their mAbs improved the developability properties, including increased Tm/Tagg (from nanoDSF) and purity (SEC)

4. A rationally-designed mAb subsequently had one key residue substituted with different amino acids; self-interaction (kD using DLS) and viscosity measurements were critical to determine that all the variants had less favorable properties

See how Prometheus Panta helped this group evaluate some of these developability parameters here.
 

View Publication

Topics: Prometheus, nanoDSF, Antibodies, Biologics, Publications

Previous Video
Prometheus Application Academy | PR.Stability Analysis software Advanced Options tutorial
Prometheus Application Academy | PR.Stability Analysis software Advanced Options tutorial

Anyone who has ever worked in a lab has heard the same refrain in some context or another – “We need more d...

Up next
Zoom in on your results using Regions of Interest in PR.Stability Analysis software
Zoom in on your results using Regions of Interest in PR.Stability Analysis software

PR.Stability Analysis software has a great new feature—Region of Interest—to help you develop that much-nee...

Ready to tackle your challenging stability characterizations?

Discover tools