A novel anti-platelet aggregation target of chinensinaphthol methyl ether and neojusticin B obtained from Rostellularia procumbens (L.) Nees

 

Songtao Wu, Yanfang Yang, Bo Liu, Zhoutao Xie, Weichen Xiong, Pengfei Hao, Wenping Xia,
Yuan Sun, Zhongzhu Ai, and Hezhen Wu

Journal of Enzyme Inhibition and Medicinal Chemistry
2019; 34(1): 999–1009 doi:10.1080/14756366.2019.1609468

Abstract

This study explored the possible bioactive ingredients and target protein of Rostellularia procumbens (L.) Nees. The results of optical turbidimetry revealed that the ethyl acetate extraction obtained from R. procumbens (L.) Nees could inhibit platelet aggregation. Gene chip was used to investigate differentially expressed genes. According to the results of the gene chip, the targets of compounds isolated from the ethyl acetate extraction were predicted by network pharmacology. Computational studies revealed that chinensinaphthol methyl ether and neojusticin B may target the integrin αIIbβ3 protein. The results of Prometheus NT.48 and microscale thermophoresis suggested that the molecular interactions between the two compounds with purified integrin αIIbβ3 protein in the optimal test conditions were coherent with the docking results. To our best knowledge, this is the first report to state that chinensinaphthol methyl ether and neojusticin B target the integrin αIIbβ3 protein.

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Topics: Plant proteins, Monolith – MicroScale Thermophoresis, MST, Proteins, Prometheus, nanoDSF, Publications

 

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