Structural basis of egg coat-sperm recognition at fertilization

 

Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L

Cell
2017 vol: 169 (7) pp: 1315-1326.e17

Introduction

Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1–3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.

View Publication

Topics: High affinity, Measure binding affinity, Monolith – Microscale Thermophoresis, Peptides, Proteins, Publications

 

 

Previous Article
Detection and characterization of small molecule interactions with fibrillar protein aggregates using Microscale Thermophoresis
Detection and characterization of small molecule interactions with fibrillar protein aggregates using Microscale Thermophoresis

Up next
Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy
Conditional internalization of PEGylated nanomedicines by PEG engagers for triple negative breast cancer therapy

×

Sign up to receive
the latest NanoTemper news, product updates, event announcements and more

First Name
Last Name
Company Name
State
Province
State
Region
State
Canton
Agree to Subscribe & Privacy Policy*
*I have fully read, understood and agree to the Privacy Policy. I accept the storing and processing of my personal data by NanoTemper as described in the Privacy Policy.

By completing this form, you provide us consent to contact you with educational content, news and information about our products, services and events. You may unsubscribe at any time.
Thank you!
Error - something went wrong!