Li X, Hao G, Wang Q, Chen Z, Ding Y, Yu L, Hu D, Song B.
2018 Mar 1;8(1):3890. doi: 10.1038/s41598-018-21952-3
Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein-protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 - the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism for the ripening inhibition.
Topics: Plant proteins, Monolith – MicroScale Thermophoresis, MST, Proteins, Publications