Rational design of a bisphenol A aptamer selective surface-enhanced Raman scattering nanoprobe

 

Haley L. Marks, Michael V. Pishko, George W. Jackson, and Gerard L. Coté

Analytical Chemistry
2014 vol: 86(23) pp: 11614–11619 doi: 10.1021/ac502541v

Abstract

Surface-enhanced Raman scattering (SERS) optical nanoprobes offer a number of advantages for ultrasensitive analyte detection. These functionalized colloidal nanoparticles are a multifunctional assay component. providing a platform for conjugation to spectral tags, stabilizing polymers, and biorecognition elements such as aptamers or antibodies. We demonstrate the design and characterization of a SERS-active nanoprobe and investigate the nanoparticles’ biorecognition capabilities for use in a competitive binding assay. Specifically, the nanoprobe is designed for the quantification of bisphenol A (BPA) levels in the blood after human exposure to the toxin in food and beverage plastic packaging. The nanoprobes demonstrated specific affinity to a BPA aptamer with a dissociation constant Kd of 54 nM, and provided a dose-dependent SERS spectra with a limit of detection of 3 nM. Our conjugation approach shows the versatility of colloidal nanoparticles in assay development, acting as detectable spectral tagging elements and biologically active ligands concurrently.

View Publication

Topics: Aptamers, Nucleotide/chemical synthesis, Aptamers, Nucleotide/chemistry, Benzhydryl Compounds/blood, Drug Design, Humans, Molecular Structure, Nanoparticles/chemistry, Phenols/blood, Spectrum Analysis, Raman, Surface Properties, Monolith–MicroScale Thermophoresis, MST, Proteins, Publications

Previous Article
Architecture and ssDNA interaction of the timeless-tipin-RPA complex
Architecture and ssDNA interaction of the timeless-tipin-RPA complex

Up next
Dye label interference with RNA modification reveals 5-fluorouridine as non-covalent inhibitor
Dye label interference with RNA modification reveals 5-fluorouridine as non-covalent inhibitor

×

Sign up to receive
the latest NanoTemper news, product updates, event announcements and more

First Name
Last Name
Company Name
State
Province
State
Region
State
Canton
Agree to Privacy Policy*
*I have fully read, understood and agree to the Privacy Policy. I accept the storing and processing of my personal data by NanoTemper as described in the Privacy Policy.

By completing this form, you provide us consent to contact you with educational content, news and information about our products, services and events. You may unsubscribe at any time.
Thank you!
Error - something went wrong!