Quantifying CBM carbohydrate interactions using Microscale Thermophoresis

 

Wu H, Montanier C, Dumon C

Methods in Molecular Biology
2017 vol: 1588

Abstract

MicroScale Thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here, we describe the application of MST in quantifying two CBM-carbohydrate interactions, a CBM3a toward cellulose nanocrystals and a CBM4 against xylohexaose.

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Topics: Ligand induced fluorescence change, Measure binding affinity, Monolith – Microscale Thermophoresis, Proteins, Publications, Saccharides

 

 

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