ESCRT-III controls nuclear envelope reformation

 

Yolanda Olmos, Lorna Hodgson, Judith Mantel, Paul Verkade & Jeremy G. Carlton

Nature
2015 vol: 522 pp: 236–239 doi:10.1038/nature14503

Abstract

During telophase, the nuclear envelope (NE) reforms around daughter nuclei to ensure proper segregation of nuclear and cytoplasmic contents. NE reformation requires the coating of chromatin by membrane derived from the endoplasmic reticulum, and a subsequent annular fusion step to ensure that the formed envelope is sealed. How annular fusion is accomplished is unknown, but it is thought to involve the p97 AAA-ATPase complex and bears a topological equivalence to the membrane fusion event that occurs during the abscission phase of cytokinesis. Here we show that the endosomal sorting complex required for transport-III (ESCRT-III) machinery localizes to sites of annular fusion in the forming NE in human cells, and is necessary for proper post-mitotic nucleo-cytoplasmic compartmentalization. The ESCRT-III component charged multivesicular body protein 2A (CHMP2A) is directed to the forming NE through binding to CHMP4B, and provides an activity essential for NE reformation. Localization also requires the p97 complex member ubiquitin fusion and degradation 1 (UFD1). Our results describe a novel role for the ESCRT machinery in cell division and demonstrate a conservation of the machineries involved in topologically equivalent mitotic membrane remodelling events.

View Publication

Topics: Monolith – MicroScale Thermophoresis, MST, Proteins, Publications

 

 

Previous Article
Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling
Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling

Up next
A plasmodium falciparum bromodomain protein regulates invasion gene expression
A plasmodium falciparum bromodomain protein regulates invasion gene expression

×

Sign up to receive
the latest NanoTemper news, product updates, event announcements and more

First Name
Last Name
Company Name
State
Province
State
Region
State
Canton
Agree to Privacy Policy*
*I have fully read, understood and agree to the Privacy Policy. I accept the storing and processing of my personal data by NanoTemper as described in the Privacy Policy.

By completing this form, you provide us consent to contact you with educational content, news and information about our products, services and events. You may unsubscribe at any time.
Thank you!
Error - something went wrong!