Aptatope mapping of the binding site of a progesterone aptamer on the steroid ring structure

Skouridou V, Schubert T, Bashammakh A, El-Shahawi M, Alyoubi A, O’Sullivan C

Analytical Biochemistry
2017 vol: 531 pp: 8-11

Abstract

In this work we report the mapping of the binding site of the only progesterone aptamer published to date, in an approach referred to as aptatope mapping. By linking the binding data obtained from microscale thermophoresis analysis to the structural differences on the ring structure of a range of steroids, we elucidated the moieties involved in aptamer-progesterone binding. This approach can be further exploited for the characterization of aptamer specificity and ultimately facilitate the development of aptamer-based assays depending on the desired specificity.

View Publication >

Topics: Aptamers, High affinity, Measure binding affinity, Monolith – Microscale Thermophoresis, Organic solvents, Proteins, Publications, Small molecules

 

Previous Article
Biophysical analysis of arabidopsis protein-only RNase P alone and in complex with tRNA provides a refined model of tRNA binding
Biophysical analysis of arabidopsis protein-only RNase P alone and in complex with tRNA provides a refined model of tRNA binding

Up next
Detection and characterization of small molecule interactions with fibrillar protein aggregates using Microscale Thermophoresis
Detection and characterization of small molecule interactions with fibrillar protein aggregates using Microscale Thermophoresis

×

Sign up to receive
the latest NanoTemper news, product updates, event announcements and more

First Name
Last Name
Company Name
State
Province
State
Region
State
Canton
Agree to Subscribe & Privacy Policy*
*I have fully read, understood and agree to the Privacy Policy. I accept the storing and processing of my personal data by NanoTemper as described in the Privacy Policy.

By completing this form, you provide us consent to contact you with educational content, news and information about our products, services and events. You may unsubscribe at any time.
Thank you!
Error - something went wrong!