An evaluation assay for thymine–mercuric–thymine coordination in the molecular beacon-binding system based on microscale thermophoresis

 

Jia Ma, Nan Liu, Li Li, Xinhua Ma, Xiaoli Li, Yanan Liu, Ya Li, Zhijiang Zhou & Zhixian Gao

Sensors and Actuators B: Chemical
2017 vol: 252 pp: 680-688

Abstract

A novel evaluation assay for molecular beacon (MB)-binding system was developed by microscale thermophoresis (MST) and thymine–mercuric–thymine coordination chemistry which was applied for the detection of mercury (II) ion (Hg2+) upon opening the hairpin and facilitating fluorescence recovery through binding with suitable mismatched target. The affinities between the loop of MBs and the complementary ssDNAs in the binding system were varied, determined and optimized by MST in the presence or absence of Hg2+. It suggested that the relative low background could be obtained when the number of mismatches of ssDNA was four and in the present of Hg2+ displayed strong recovery of fluorescence. The lowest detection limit of Hg2+ was 3 nM which was below the maximum contaminant level defined by U.S. Environmental Protection Agency (10 nM). The working range was 5–320 nM with negligible possible interference from the different background composition of water samples by optimization of the binding conditions. Through evaluating by MST, the molecular interaction mechanism was elaborated and the rapid, sensitive and selective detection of Hg2+ was achieved by the designed MB-binding system. It herein has great potential for appraising and analyzing molecular interaction among the interactants.

View Publication

Topics: Ions, Monolith – MicroScale Thermophoresis, MST, Proteins, Publications

 

 

Previous Article
Fragment-based approach to identify IDO1 inhibitor building blocks
Fragment-based approach to identify IDO1 inhibitor building blocks

Up next
The molecular basis of phosphite and hypophosphite recognition by ABC-transporters
The molecular basis of phosphite and hypophosphite recognition by ABC-transporters

×

Sign up to receive
the latest NanoTemper news, product updates, event announcements and more

First Name
Last Name
Company Name
Country
State
Province
State
Region
State
Canton
Agree to Subscribe & Privacy Policy*
*I have fully read, understood and agree to the Privacy Policy. I accept the storing and processing of my personal data by NanoTemper as described in the Privacy Policy.

By completing this form, you provide us consent to contact you with educational content, news and information about our products, services and events. You may unsubscribe at any time.
Thank you!
Error - something went wrong!