1 6
A P P L I C A T I O N N O T E
Figure 3: Thermal unfolding analysis of WbaP stabilized by AASTY 6-55 nanodisc.
10
15.849
Cumulant
Radius
[nm]
91
92
93
94
Turbidity
[mAU]
-12
-10
-8
-6
350
nm
First
Derivative
600
800
1000
1200
350
nm
[counts
*
mm]
30 40 50 60 70 80 90
Temperature [°C]
10
31.623
100
316.228
1000
3162.278
Cumulant
Radius
[nm]
85
90
95
100
105
110
115
120
125
Turbidity
[mAU]
0.7
0.72
0.74
0.76
0.78
0.8
Ratio
350
nm
/
330
nm
30 40 50 60 70 80 90
Temperature [°C]
AASTY11-45
AASTY6-50
Figure 4: Thermal unfolding analysis of PglB stabilized by AASTY 11-45 (PglB 12)
and AASTY 6-50 (PglB 13) nanodiscs across a thermal ramp.
