Application Notes

Optimization of membrane proteins for cryo_EM

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1 7 A P P L I C A T I O N N O T E Figure 5: Thermal unfolding analysis of Hp PglC stabilized by SMALP BZ35, AASTY 6-45, and AASTY 6-55 nanodiscs. 10 31.623 100 316.228 Cumulant Radius [nm] 90 100 110 120 130 Turbidity [mAU] 0 0.002 0.004 0.006 Ratio First Derivative 0.76 0.8 0.84 0.88 Ratio 350 nm / 330 nm 40 50 60 70 80 90 Temperature [°C] SMALPBZ35 AASTY6-45 AASTY6-55

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