Application Notes

Optimization of membrane proteins for cryo_EM

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1 5 A P P L I C A T I O N N O T E Table 2 Thermal stability of WbaP, PglB, and PglC nanodiscs Index Copolymer WbaP PglB Hp PglC T onset r H (°C) IP 350 nm (°C) T onset r H (°C) T onset Turbidity (°C) T onset r H (°C) IP ratio (°C) 1 SMA25 (SMA300) 75.0 N.A. 69.2 80.2 69.8 68.0 2 SMA30 (SMA200) 69.0 N.A. 77.2 >90 >75 59.5 3 SMA40 (SMA140) 4 DIBMA 10 5 Sulfo-DIBMA 6 Sulfo-SMA 7 SMA BZ25 59.7 76.2 49.9 55.4 8 SMALP BZ30 61.3 81.8 51.5 55.7 9 SMALP BZ35 52.2 N.A. 65.5 81.8 51.1 56.8 10 SMALP BZ40 N.A. N.A. 50.1 80.0 48.8 57.2 11 AASTY 6-45 65.7 57.5 46.0 50.2 51.1 48.2 12 AASTY 11-45 N.A. 51.8 87.3 >95 69.6 50.6 13 AASTY 6-50 68.5 55.5 56.0 60.6 47.3 48.3 14 AASTY 11-50 (1) 80.4 >95 62.3 50.2 15 AASTY 6-55 74.7 53.9 62.0 86.2 51.5 48.9 16 AASTY 11-50 (2) 79.7 N.A. 73.0 >90 59.6 47.0

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