Measuring challenging interactions between various types of
molecules at times requires a different approach. So having
two different modalities in the same instrument helps you
cover all the types of interactions you encounter.
Dianthus is the first affinity screening platform to
use Spectral Shi
The notion of spectral shi isn't new, but Dianthus is the first
platform that uses it to derive affinity constants.
The process is actually pretty simple: label one binding partner
with a fluorophore, then mix a fixed amount of it with a dilution
series of the ligand. A er excitation at 590nm of the mix, the
binding events are detected as shi s of spectra towards either
blue or red. The detection of spectral shi s is achieved through
dual-wavelength detection at exactly 650nm and 670nm in an
isothermal environment — this makes Dianthus so precise and
able to detect very subtle shi s.
Then the K
d
is calculated by plotting the ratios of the
fluorescence intensities from the two wavelengths against the
ligand concentration.
Ensure your success with these
two biophysical modalities
10
-11
10
-10
10
-9
10
-8
10
-7
10
-6
10
-5
0.96
0.98
1.00
1.02
1.04
1.06
1.08
Ratio
670
nm
/650
nm
Ligand concentration (M)
K
d
650
670
Emission wavelength (nm)
Spectral Shi
690
630
Fluorescence
intensity
