Use TRIC — a proven technology that's been
around for over 10 years that compliments
Spectral Shi
Quantifying molecular interactions with TRIC is done
by labeling your target molecule with a fluorescent dye
and mixing it with your ligand. Then, a very precise
and brief laser-induced temperature change is applied,
which amplifies the variation in fluorescence intensity
caused by the ligand binding to your target.
The K
d
is calculated by plotting the change in
fluorescence against the ligand concentration.
Relative
Fluorescence
Time (s)
0 1 2 3 4 5
0 .8 0
0 .8 5
0 .9 0
0 .9 5
1 .0 0
1 .0 5
F
norm
(‰)
Ligand concentration (M)
1 E 1 E 1 E 1 E
8 5 0
8 6 0
8 7 0
8 8 0
8 9 0
9 0 0
9 1 0
1 E
K
d
