APPLICATION NOTE
Bernhard Valldorf
1
, Adrian Fricke
2
, Stefanie Kall
3
1
Principlal scientist, Merck Healthcare KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany
2
PhD student, Institute for Physiological Chemistry, Philipps-Universität Marburg
3
NanoTemper Technologies, Germany
Abstract
Self-interaction of therapeutic proteins has an impact on the ultimate success of a
biologic candidate. During the development process, candidates are o en assayed
at concentrations that differ from that of their final delivery form. Understanding how
therapeutic proteins behave at higher concentrations is crucial for ensuring their ultimate
success in the clinic. Dynamic (DLS) and static (SLS) light scattering techniques help
researchers determine and optimize the self-interaction behavior of protein-based
therapeutics ahead of scale-up and manufacture. Here we show how the Prometheus
Panta measures self-interaction of proteins and allows researchers to prioritize
candidates and improve outcomes.
Introduction
Light-scattering techniques give researchers important insight into the biophysical
characteristics of their therapeutic protein candidates. One example that is of concern
to researchers is self-interaction. Therapeutic proteins are o en delivered to patients
at much higher concentrations than seen during the developmental process, and this
can lead to promising candidates failing in the clinical stage a er lengthy and costly
development1. Researchers have small batches of candidates to use during development,
and it is not until they pass a series of qualifying tests that they will enter into scale-up
Use of self-interaction parameters derived from
light scattering experiments for better developability
assessment of biologic therapeutics
