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7
APPLICATION NOTE
DLS plate reader: Histidine
pH 5.5 SLS stability predictor
70,000,000
60,000,000
50,000,000
40,000,000
30,000,000
20,000,000
10,000,000
0
0.0 5.0 10.0 15.0 20.0
Plate 1 His 5.5
Concentration (mg/ml)
Intensity
(cnt/s)
Plate 2 His 5.5
3E+06x + 1E+07
R
2
= 0.9742
y =
2E+06x + 1E+07
R
2
= 0.9323
y =
Panta: Histidine
pH 5.5 SLS stability predictor
14,000,000
12,000,000
10,000,000
8,000,000
6,000,000
4,000,000
2,000,000
0
0.0 5.0 10.0 15.0 20.0
Panta 1 His 5.5
Concentration (mg/ml)
Intensity
(cnt/s)
Panta 2 His 5.5
762726x + 1E+06
R
2
= 0.991
y =
792957x + 1E+06
R
2
= 0.991
y =
Figure 5: Example plots for determining deviation from linearity for plate reader (le ) vs Panta (right). The Panta exhibits exemplary repeatability between
measurements.
Though the Panta gave systematically lower SLS stability prediction values, it is also of
note to examine the reproducbility between measurements. In Figure 5, we see that the
linear regression lines from Panta demonstrate great agreement with each other, as well
as exceedingly high R
2
values, indicating exemplary linear fits.
