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6
APPLICATION NOTE
Prometheus Panta allows measurement of the deviation from linearity using an absolute
measurement of scattered light. The EAP instrument used here was not yet calibrated
for full SLS analysis and molecular weight measurements, but scattering results in
Figure 4 show that the deviation from linearity for scattered light lines up well with other
instruments in the field.
mAb1 SLS deviation from linearity (%)
0.0
-20.0
-40.0
-60.0
-80.0
-100.0
-120.0
-140.0
-160.0
Citrate pH 5.5
Panta Plate reader Panta Plate reader
His pH 5.5
-101.71
-115.07
-56.61
-24.95
Deviation
from
linearity
(%)
Figure 4: Comparison of SLS deviation from linearity for Prometheus Panta vs plate reader system. The Panta gave
systematically lower values compared to the alternative, but with less standard deviation.
