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4
APPLICATION NOTE
Figure 1 shows that candidate molecules 1 and 2 exhibited negative k
D
for all buffer
systems. Histidine-based buffers showed the most success for molecules 3-5. This
information allows not only the selection of the ideal molecule to move forward with
during development, but also the optimal buffer conditions for storage and assays.
These molecules had been previously investigated for their self-interaction behavior
using a plate-reading system. As the goal of the EAP project was to ensure that Panta gave
accurate measurements that agree with current literature values, the k
D
values measured
on each system were compared. Figure 2 demonstrates that the results obtained from the
Panta are in good agreement with those from previous plate reader experiments.
mAb1 k
D
(mL/g)
0.0
-5.0
-10.0
-15.0
-20.0
-25.0
-30.0
Citrate pH 5.5
Panta Plate reader Panta Plate reader
His pH 5.5
-2.60
-2.26
-26.31
-25.38
k
D
(ml/g)
Figure 2: Representative comparison of
DLS k
D
values between Prometheus Panta
(EAP) and a plate reader system. The
values agree within standard deviation;
the Panta demonstrated less variation
between triplicates.
The k
D
values obtained from Panta DLS quickly and efficiently show which candidates
and formulations have optimal self-association values to decrease the potential for
aggregation at higher concentrations. The values obtained from the Panta also line up
well with previously published data, demonstrating how it can be reliably instituted into
existing workflows.
