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A P P L I C A T I O N N O T E
Figure 2: . Automated Dianthus uHTS workflow. A. Schematic of Dianthus uHTS integrated into an automated
screening platform. B. Assay workflow for single point screen.
RESULTS
MEK1 Labeling with Spectral Shi Optimized Dye L021. MEK1 was successfully labelled, with a
final concentration of 3 µM aer removal of free dye, and a degree of labeling of 0.8.
Integration into an automated Screening Platform. The Dianthus uHTS was integrated into an
internally built screening platform, where plate movement was performed by a ThermoFisher Orbit-
er RS1 arm and controlled with the ThermoFisher Momentum Scheduler soware (Figure 2A). The
Dianthus uHTS comes with an API based on Google Remote Procedure Call (gRPC) framework. Due to
the simplicity of the measurement mode of the Dianthus, a simple command line driver was written
in python to open and close the gate, and start a measurement, where measurement parameters
(well choice and excitation power) are passed from Momentum to the command line.
The workflow for a Dianthus uHTS single point screen and affinity screen is similar (Figure 2B). Dian-
thus uHTS 1536 well plates were pre-dispensed at 3x concentration with the compound library or di-
lution series using an Integra Assist+ from source plates with pre-dilutions before being loaded onto
the automated platform, where labelled MEK1 was added to a final concentration of 5 nM, followed
by mixing, centrifugation, sealing, incubation, a further centrifuge step before final measurement in
the Dianthus uHTS device.
