9
A P P L I C A T I O N N O T E
Equation. 4
Equation. 5
Single Point n=2 Assay Normalization was performed using the robust Z' score method [14] per
plate using Equation 6, comparing each value with the median of the DMSO reference (x̃ ). Spectral
Shi measurements can be either red or blue shied, but in the context of screening, absolute robust
z score values are reported for ease of interpretation.
Equation. 6
Estimation of K
d
was performed as reported in[1]. Conversion to picometer spectral shi units was
achieved using Equation 7, where s is ratio signal for a concentration point, r_
"unbound"
the fit coefficient
for the unbound state from the estimation of K
d
.
Equation. 6
Figure Generation. Plots and data visualizations were generated in a Jupyter Notebook (v5.7.2),
using Python (v3.11.7), with the following packages: numpy (v2.2.1), pandas (v1.5.3), matplotlib
(v3.8.0), seaborn (v0.13.2) and matplotlib-venn (v1.1.1) and exported as png. Composite figures were
subsequently generated in Microso PowerPoint.
