Application Notes

Optimization of membrane proteins for cryo_EM

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1 0 A P P L I C A T I O N N O T E Table 1 Hydrodynamic radius of the major peak in DLS analysis of WbaP, PgIB and Hp PglC nanodiscs Index Copolymer r H (nm) WbaP PglB Hp PglC 1 SMA25 (SMA300) 7.46 ± 0.48 9.68 ± 0.45 9.59 ± 0.32 2 SMA30 (SMA200) 9.16 ± 0.72 8.71 ± 0.34 6.19 ± 1.54 3 SMA40 (SMA140) 32.18 ± 3.09 24.19 ± 2.83 22.18 ± 1.08 4 DIBMA 10 29.39 ± 2.39 29.10 ± 7.57 22.42 ± 2.5 5 Sulfo-DIBMA 30.07 ± 1.41 24.33 ± 2.47 23.29 ± 1.11 6 Sulfo-SMA 36.99 ± 2.34 27.23 ± 1.35 25.22 ± 1.8 7 SMALP BZ25 28.68 ± 10.76 8.48 ± 2.07 8.26 ± 1.53 8 SMALP BZ30 20.19 ± 4.59 9.91 ± 0.64 7.90 ± 2.52 9 SMALP BZ35 12.73 ± 1.19 9.73 ± 1.26 13.22 ± 4.32 10 SMALP BZ40 10.95 ± 0.49 10.21 ± 0.48 8.39 ± 0.79 11 AASTY 6-45 11.4 ± 0.47 10.84 ± 0.18 8.6 ± 0.43 12 AASTY 11-45 13.27 ± 4.39 8.44 ± 0.5 7.86 ± 1.72 13 AASTY 6-50 10.54 ± 2.08 8.92 ± 0.41 7.49 ± 1.17 14 AASTY 11-50 (1) 24.36 ± 7.89 8.47 ± 1.57 13.28 ± 4.18 15 AASTY 6-55 11.69 ± 2.51 8.68 ± 0.46 7.80 ± 1.73 16 AASTY 11-50 (2) 12.83 ± 3.20 8.35 ± 0.36 6.93 ± 1.33

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