1 1
A P P L I C A T I O N N O T E
0
2
4
6
8
10
12
Relative
probability
1 10 100 1000
Radius [nm]
SMA25(SMA300)
SMA30(SMA200)
SMALPBZ35
SMALPBZ40
AASTY6-45
AASTY11-45
AASTY6-50
AASTY6-55
AASTY11-50(2)
0
2
4
6
8
10
12
14
Relative
probability
1 10 100 1000
Radius [nm]
SMA40(SMA140)
DIBMA10
Sulfo-DIBMA
Sulfo-SMA
SMALPBZ25
SMALPBZ30
AASTY11-50(1)
Figure 1: Overlayed DLS size distribution plots
and cumulant radius (dash lines) for WbaP solubilized with 16 different copolymers.
Figure 2: SDS-PAGE gel of WbaP solubilized in each polymer. Each lane was loaded with 8 µL
of eluate from strep resin. Lane 2 (SMA30), Lane 10 (SMALP BZ40), and Lane 15 (AASTY 6-55)
are highlighted with arrows.
SMA30
6.84 nm
SMALP BZ40
8.1 nm
AASTY 6-55
10.96 nm
A
B
