7
A P P L I C A T I O N N O T E
PROTEIN 1: WBAP COPOLYMER SCREEN
WbaP is a prototypic member of the large monotopic phospho-
glycosyl transferase superfamily from Salmonella enterica. This
protein catalyzes the transfer of galactose-1-phosphate onto un-
decaprenyl phosphate (Und-P) during the initial stage of lipopoly-
saccharide O-antigen biosynthesis and is a potential target for an-
tibiotic development
(2)(3)
. This class of bacterial proteins contains
several subdomains, including a transmembrane four-helix bun-
dle, a soluble nucleotide sensing domain, and a membrane-bound
catalytic domain containing a re-entrant membrane helix
(4)
.
The first cryo-EM structure of WbaP required extensive optimiza-
tion of copolymer-based solubilization, ultimately leading to the
development of this protocol.
SMA
AASTY
Class of copolymers included in the Cube Synthetic Nanodisc screening Kit MAXI
