6
A P P L I C A T I O N N O T E
The copolymers of the NativeMP
TM
Platform are commercially available
and have been used for membrane protein studies all over the world.
In particular, the group of Prof. Barbara Imperiali and postdoctoral
researcher Dr. Greg Dodge at MIT had great success utilizing this platform.
As illustrated in this application note, the combination of the NativeMP
TM
platform with the Prometheus Panta provided an optimal workflow for
the prokaryotic membrane protein targets from the Imperiali group. The
experiments shown in this application note have been performed by the
Imperiali lab at MIT. More examples can be viewed in the webinar of Greg
Dodge on the Cube Biotech Website.
Cube Biotech and the Imperiali Group validated that these
parameters together allow rapid screening for optimal copolymers
to solubilize a variety of membrane protein types, which was
corroborated by information from SEC and SDS-PAGE. We
anticipate that this workflow will be of general utility to the greater
membrane protein community, especially for those interested in
applications such as structural biology.
