Monolith X uses Spectral Shi Technology to quantify
molecular interactions between a target and ligand. When
the target is labeled with a fluorophore, it gives a particular
emission spectrum. If a ligand binds, the fluorophore's
chemical environment is changed, causing a blue-shi or red-
shi or broadening of the emission peak (top).
Fluorescence is recorded and a 670nm/650nm ratio is
calculated and plotted against the ligand concentration
(bottom). This ratiometric measurement is used to derive the
affinity constant (K
d
) which is automatically determined at the
end of each run without additional and lengthy data analysis.
Use Spectral Shift Technology
to evaluate interactions
650
670
Emission wavelength (nm)
Spectral Shi
690
630
Fluorescence
intensity
10
-11
10
-10
10
-9
10
-8
10
-7
10
-6
10
-5
0.96
0.98
1.00
1.02
1.04
1.06
1.08
Ratio
670
nm
/650
nm
Ligand concentration (M)
K
d
