MST is sensitive to the presence of aggregates in your sample
and it provides a way to gain insights beyond a K
d
.
With MST and Spectral Shi in the same instrument, you have
two modalities that complement each other so that you can
get further insights about your interactions.
To quantify molecular interactions with MST, you start by
labeling your target molecule with a fluorescent dye and
mixing it with your ligand. Then, a very precise and brief
laser-induced temperature change is applied, which amplifies
the variation in fluorescence intensity caused by the ligand
binding to your target (top figure).
The K
d
is calculated by plotting the change in fluorescence
against the ligand concentration (bottom figure)
Use MST Technology to
understand aggregation
-5 5 10 15 20
Time (seconds)
750
800
850
900
950
1000
F
norm
(‰)
0
1 10 100 1000
Ligand (nM)
810
820
830
840
850
F
norm
(‰)
K
d
