Characterize your PROTAC hook effect
with flexible and in-solution affinity
measurements for results you can trust
Once the screening for warheads and ligase ligands is over and you finally have
fully assembled PROTAC candidates, you need to characterize the ternary complex
hook effect. In order to select candidates that enhance cooperativity — a proven
approach that mitigates the extent of the hook effect by favoring ternary complex
stability — it's important to determine the biophysical parameters of the ternary
complex.
Dianthus with Spectral Shi technology — a plate-based affinity screening platform
that handles the throughput needed for hit identification and lead validation —
also excels in the characterization of ternary complexes and the hook effect with
these advantages:
1 2 3
Get a flexible assay setup
— you have the option to
label the POI or E3 ligase
when you measure binding
affinities
Measure affinities in
solution — so you don't
stress out about having
to immobilize ternary
complex components
Generate data that agree
with a mathematical
model for three-component
binding equilibria,
so you feel confident
about your results
