Get data that agrees
with the fit model currently used to
study ternary complexes
It's hard to make decisions with data that can't give you clear answers
on whether hits are real, or binding affinities are precise.
Dianthus consistently outputs highly reproducible measurements
that align to fit the model that best represents the interactions
in binary and ternary complexes.
The interaction between target Brd2 and PROTAC MZ1 was measured
and the data was in very good agreement with the fit model*
to calculate the K
d
(18.8 nM) and the cooperativity value (a = 9.3).
From these parameters, the affinity constant of the ternary complex
Brd2+MZ1+Ligase VCB was determined (K
d
= 2.02 nM).
10
-2
10
-1
10
0
10
1
10
2
10
3
10
4
0.92
0.96
1.00
1.04
1.08
1.12
Ratio
670nm/
650nm
MZ1 (nM)
Binary complex BRD2 + MZ1
Ternary complex BRD2 + MZ1 + VCB
*Douglas et al J Am Chem Soc 2013, 135:6092-6099
