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The biologics researcher's guide to DLS

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42 What can I do with a high PDI sample? If your measured PDI indicates polydispersity, start by looking at the intensity distribution. This will help tell you if there are multiple species of different sizes, how large those multiple species are, and how wide the distribution around a certain size is. 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 1 10 100 PDI-0.17 Relative Frequency Radius [nm] 1000 0 0.02 0.04 0.06 0.08 1 10 100 PDI=0.29 Relative Frequency Radius [nm] 1000 Example of increased PDI centred around a single species

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