Issue link: https://resources.nanotempertech.com/i/1309390
TECHNICAL NOTE 4 ©2020 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. routinely achievable with both formats of the sample tray. Obtaining the same results independent of the sample tray format used gives the users confidence that no matter what throughput they choose, they can trust Prometheus Panta's measurements repeatability. Conclusion The results presented here demonstrate the sizing accuracy and intra-assay precision of the DLS optical module in Prometheus Panta. With its outstanding accuracy, it reliably determines the size of proteins Figure 2 shows the mean r H values for all 48 samples loaded in single capillaries from 10 independent measurements for both NISTmAb and Lysozyme. The data demonstrates the strong homogeneity across the entire sample tray of the instrument, which is very important to ensure intra-assay comparability of sample data Table 2 gives an overview of the average variation of DLS measurements for both the 48 single capillary sample positions and the 24 sample positions on a capillary chip, from 10 individual measurements. The values highlight the outstanding repeatability and precision 10 2 0 30 4 0 5 0 0 2 4 6 8 1 0 r [ n m ] H NIST mAb L yso zym e Capillary position Figure 2: Precision of Prometheus Panta's DLS optical module over all 48 sample positions for Lysozyme (0.5 mg/mL) and NISTmAb (0.1 mg/mL). Dots represent mean r H , error bars represent the standard deviation (n=10). Table 2: Mean r H values of NISTmAb and Lysozyme in 48 single capillaries or in 24 capillaries on a chip. Sample Tray Format r H * NISTmAb r H * Lysozyme 48 single capillaries 4.9 ± 0.1 nm 1.9 ± 0.1 nm 24 capillaries on chip 4.9 ± 0.2 nm 1.7 ± 0.1 nm * standard deviation from 10 independent measurements and other molecules. It is the first instrument that can characterize samples with nanoDSF, backreflection technology, and DLS simultaneously throughout the entire thermal ramp on as many as 48 samples. Whether this characterization serves as in-depth stability evaluation or as a quality control step ahead of costly large-scale work, scientist can count on high-resolution, domain-specific stability characterization of candidate molecules, which will help them confidently make better-informed decisions.