Technical Notes

Site-specific covalent labeling of SNAP-tagged proteins for the measurement of binding affinities

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SNAP-tag® is a registered trademark of New England Biolabs, Inc. Monolith and NanoTemper are registered trademarks of NanoTemper Technologies GmbH, Munich, Germany. ©2020 NanoTemper Technologies, Inc. South San Francisco, CA, USA. All Rights Reserved. TE-MO-002-01 nanotempertech.com References 1 Bartoschik T, Galinec S, Kleusch C, et al. Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis. Sci Rep. 2018;8(1):4977. doi:10.1038/s41598-018-23154-3 2 Jongsma MA, Litjens RHGM. Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address. Proteomics. 2006;6(9):2650-2655. doi:10.1002/pmic.200500654 3 Keppler A, Pick H, Arrivoli C, Vogel H, Johnsson K. Labeling of fusion proteins with synthetic fluorophores in live cells. 2004;101(27):9955-9959. 4 Keppler A, Kindermann M, Gendreizig S, Pick H, Vogel H, Johnsson K. Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro. Methods. 2004;32(4):437-444. doi:10.1016/j.ymeth.2003.10.007 5 Maurel D, Comps-Agrar L, Brock C, et al. Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: Application to GPCR oligomerization. Nat Methods. 2008;5(6):561-567. doi:10.1038/nmeth.1213 6 Meyer BH, Segura JM, Martinez KL, et al. FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci U S A. 2006;103(7):2138-2143. doi:10.1073/pnas.0507686103 7 Pick H, Jankevics H, Vogel H. Distribution Plasticity of the Human Estrogen Receptor α in Live Cells: Distinct Imaging of Consecutively Expressed Receptors. J Mol Biol. 2007;374(5):1213-1223. doi:10.1016/j.jmb.2007.10.007 8 Sielaff I, Arnold A, Godin G, Tugulu S, Klok HA, Johnsson K. Protein function microarrays based on self-immobilizing and self-labeling fusion pro- teins. ChemBioChem. 2006;7(1):194-202. doi:10.1002/cbic.200500278 9 Juillerat A, Gronemeyer T, Keppler A, et al. Directed Evolution of O6-Alkylguanine-DNA Alkyltransferase for Efficient Labeling of Fusion Proteins with Small Molecules In Vivo. Curr Biol. 2003;13(Figure 2):654-658. doi:10.1016/S

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