APPLICATION NOTE
7
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0
20
40
ΔFnorm
[‰]
0.92
0.96
1.00
-1 0 1 2 3 4 5
Time [s]
Relative
Fluorescence
[-]
930
935
940
945
950
955
1x10
2
1x10
3
1x10
4
1x10
5
1x10
6
1x10
7
Fnorm
[‰]
Ligand Concentration
0.90
0.92
0.94
0.96
0.98
1.00
Relative
Fluorescence
[-]
-1 0 1 2 3 4 5
Time [s]
Fragment C18598
(A) (C)
(B) (D)
-4
-2
0
2
4
6
ΔFnorm
[‰]
0.92
0.96
1.00
-1 0 1 2 3 4 5
Time [s]
Relative
Fluorescence
[-]
930
932
934
936
938
940
942
Fnorm
[‰]
1x10
2
1x10
3
1x10
4
1x10
5
1x10
6
1x10
7
Ligand Concentration
0.90
0.92
0.94
0.96
0.98
1.00
Relative
Fluorescence
[-]
-1 0 1 2 3 4 5
Time [s]
Fragment A25749
(A) (C)
(B) (D)
Single-dose screen
(A) ΔFnorm
(B) TRIC trace
Affinity screen
(C) Fnorm dose response curve
(D) TRIC trace
Fragment
